Abstract
Quantitative proteins analysis was carried out in the hypothalamus of zebrafish following dietary exposure to the legacy pesticide dieldrin. Data were collected using iTRAQ labeling methodology and data were acquired using a hybrid quadrupole Orbitrap (Q Exactive) MS system (Thermo Fisher Scientific, Bremen, Germany). There were 3941 proteins identified in the hypothalamus of zebrafish, and these proteins comprised 23 unique expression patterns for proteins based on the three doses of dieldrin. There were 226 proteins that were regulated in one or more doses of dieldrin and 3715 proteins that were not affected. Thus, 5.7% of the proteins detected responded to the treatment. Many proteins that were differentially expressed were those found in, or associated with, the mitochondria. The proteomics data described in this article is associated with a research article, “Transcriptomic and proteomic analysis implicates the immune system and mitochondria as molecular targets of dieldrin in the zebrafish (Danio rerio) central nervous system” (A.M. Cowie, K.I. Sarty, A. Mercer, J. Koh, K.A. Kidd, C.J. Martyniuk, submitted) [1], and serves as a resource for researchers working in the field of pesticide exposures and protein biomarkers.
Highlights
The pesticide dieldrin disrupts proteins related to oxidative respiration and mitochondrial stress in the central nervous system
There were 3941 proteins identified in the hypothalamus of zebrafish, and these proteins comprised 23 unique expression patterns for proteins based on the three doses of dieldrin
Martyniuk, submitted) [1], and serves as a resource for researchers working in the field of pesticide exposures and protein biomarkers
Summary
Biology Pesticide neurotoxicity, proteomics, biomarkers, pathway analysis, zebrafish. There were 3941 proteins identified in the hypothalamus of zebrafish and 226 proteins that were regulated in one or more doses of dieldrin (Supplemental Data 1) These proteins comprised 23 unique expression patterns for proteins based on the three doses of dieldrin (Table 1). The remaining proteins that were not changed (3715) are indicated in group X1.The most dramatic proteomic response in terms of protein number occurred with the high feed dose, and 61 proteins were significantly decreased (Cluster XII) with 1.8 mg DLD/g d.w. feed. Proteins in this group were not changed with the two lower doses of dieldrin. Eighteen proteins involved in the respiratory chain were affected by dieldrin, for example SLC8A1 (Solute Carrier Family 8 Member A1), SLC25A12 (Solute Carrier Family 25 Member 12), IDH2 (Isocitrate Dehydrogenase (NADP( þ)) 2, Mitochondrial), MDH2 (Malate Dehydrogenase 2), LARS2 (Leucyl-TRNA Synthetase 2, Mitochondrial), NAPG (NSF Attachment Protein Gamma), Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V), and CYP2A6 among others
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