The perspective fluoroplastic porous carrier for conservation and transfer of samples of blood plasma: application of "dry drop" technique with the purpose of detection of individual fatty acids.

Abstract

It is proposed for preserving samples of biological fluids (blood plasma first of all) and mailing them to analytical laboratory to significantly modify a well-known technique of "dried blood spot" (DBS) at the expense of application of home-made fluoroplastic filtering material type MFFK-G as a porous carrier of fluid biological sample. The synthetic material contains no chemically active molecular fragments and it has four times greater water capacity than filter and chromatographic paper. Thus, fluoroplastic porous disc of mass of 15 mg is suitable to carrying of 70-100 mkl (instead of 15-20 mkl) of fluid. The preliminary impregnation of such a disc with solution of antioxidant - 0.5% - 0.9% 2,6-di-tret-butyl-4-methyl phenol in chloroform - permits to strongly increase stability of higher poly-saturated fatty acids in applied of dry plasma sample or erythrocytic mass on the disc. In that case, time of storage of ready-made disc with dry sample under room temperature increases up to 35-40 days and time of storage in freezer - from 50-60 days to half a year and longer. The storage of dry samples in airtight bottles of vials air-blown with technical propanebutane, also increases time of storage of samples approximately on a degree. This method permits to qualitatively determine content of individual fatty acids in the fraction of etherized and non-etherized fatty acids and also total and free cholesterol in samples of biological fluids.