Abstract
The applicability of fluorescence detection in miniaturized cells using a helium—cadmium laser as the excitation source at 325 nm to open-tubular liquid chromatography has been studied. The investigated cells were fused-silica capillaries of 25 and 10 μm I.D., comprising part of the column or being coupled separately to it, and a sheath flow cell. The volume standard deviation of the various cells was found to be 0.1–2 nl which makes them suitable for the above purpose. The mass detection limits were found to be in the attogram range. However, the concentration detection limits, were found to be one to two orders of magnitude higher than those obtainable with conventional sized fluorescence detectors. This is mainly caused by the instability of the laser.
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