Abstract
Background: Membrane technology has been widely applied for protein purification. In applications for protein separation, a membrane with stable filtration performance is necessary. In this work, two types of hollow fiber membranes with different characteristic were used to study the filtration profile of bovine serum albumin. Methods: A single piece of hollow fiber module was used for ultrafiltration testing using UF0 and UFT304 membranes. Flux and rejection of BSA solution were collected based on a pressure-driven inside filtration model. Results: Ultrafiltration experiments showed that the flux of UFT304 membrane was higher than that of UF0 membrane in all applied pressure condition. Solute rejection reaches 90 and 88% for ultrafiltration of BSA solution on the operating pressure of 0.5 atm using UF0 and UFT304 membranes, respectively. Conclusion: In general, UFT304 membranes has better ultrafiltration performance for BSA separation than UF0 membranes. The UFT304 membrane has a more stable flux for up to two hours of filtration.
Highlights
Membrane technology has been widely applied for protein purification
We changed the sentence in Results part of abstract from “solute rejection was achieved up to 90% for ultrafiltration of bovine serum albumin (BSA) solution by using UF0 membrane obtained on the operating pressure of 0.5 atm” became “Solute rejection reaches 90 and 88% for ultrafiltration of BSA solution on the operating pressure of 0.5 atm using UF0 and UFT304 membranes, respectively”
This study aimed to investigate and establish the profile of BSA solution filtration using a standard PES membrane and a membrane modified with Tetronic 304 surfactant for permeability and rejection parameters
Summary
Membrane technology has been widely applied for protein purification. In applications for protein separation, a membrane with stable filtration performance is necessary. Methods: A single piece of hollow fiber module was used for ultrafiltration testing using UF0 and UFT304 membranes. Flux and rejection of BSA solution were collected based on a pressure-driven inside filtration model. Results: Ultrafiltration experiments showed that the flux of UFT304 membrane was higher than that of UF0 membrane in all applied pressure condition. Solute rejection reaches 90 and 88% for ultrafiltration of BSA solution on the operating pressure of 0.5 atm using UF0 and UFT304 membranes, respectively. Conclusion: In general, UFT304 membranes has better ultrafiltration performance for BSA separation than UF0 membranes. The UFT304 membrane has a more stable flux for up to two hours of filtration
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