Abstract
Background: Membrane technology has been widely applied for protein purification. In applications for protein separation, a membrane with stable filtration performance is necessary. In this work, two types of hollow fiber membranes with different characteristic were used to study the filtration profile of bovine serum albumin. Methods: A single piece of hollow fiber module was used for ultrafiltration testing using UF0 and UFT304 membranes. Flux and rejection of BSA solution were collected based on a pressure-driven inside filtration model. Results: Ultrafiltration experiments showed that the flux of UFT304 membrane was higher than that of UF0 membrane in all applied pressure condition. Solute rejection was achieved up to 90% for ultrafiltration of BSA solution by using UF0 membrane obtained on the operating pressure of 0.5 atm. Conclusion: In general, UFT304 membranes has better ultrafiltration performance for BSA separation than UF0 membranes. The UFT304 membrane has a more stable flux for up to two hours of filtration.
Highlights
Albumin proteins are one of the most important substances in the human body
Tetronic 304 is a non-ionic surfactant with two bonds of polyethylene oxide (PEO) and two bonds of polypropylene oxide (PPO) to ethylene diamine group
This study aimed to investigate and establish the profile of bovine serum albumin (BSA) solution filtration using a standard PES membrane and a membrane modified with Tetronic 304 surfactant for permeability and rejection parameters
Summary
Albumin proteins are one of the most important substances in the human body. Albumin maintains the balance of vascular fluid, forms new cell tissue, and functions acts as an antibody[1]. Rapid development in the field of biotechnology has allowed for the separation of proteins with safer and more efficient methods, namely purification using membrane technology (ultrafiltration, UF). This method has been reported to successfully achieve protein fractionation through UF with several advantages compared with the conventional separation using biology/ chemistry methods[4,5,6]. Tetronic 304 is a non-ionic surfactant with two bonds of polyethylene oxide (PEO) and two bonds of polypropylene oxide (PPO) to ethylene diamine group This surfactant potentially improves hydrophilic traits of the PES membrane due to its PEO group. This study aimed to investigate and establish the profile of BSA solution filtration using a standard PES membrane and a membrane modified with Tetronic 304 surfactant for permeability and rejection parameters
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