Abstract
Introduction: Even if the prevalence of intestinal parasites is high in Ethiopia, we still use only direct wet mount method for laboratory diagnosis of intestinal parasitic infections, having low sensitivity, and this significantly increases false-negative results. Therefore, the performance evaluation of three laboratory diagnostic methods is mandatory. 
 Methods: Single stool sample was collected from March 2018 to June 2018, among 211 school children, and processed using a wet mount, modified Baermann (MB), and Ritchie’s methods. The sensitivity and negative predictive values (NPVs) at 95% confidence interval and Kappa values were calculated in terms of the gold standard method (the combined result of altogether). 
 Results: The overall prevalence of intestinal parasites was 60.2%. The sensitivity and NPVs of the wet mount, MB, and Ritchie’s methods against the “Gold standard” test were 49.6% and 56.8%, 80.3% and 77.1%, and 67.7% and 68.8%, respectively. 
 Conclusions: MB showed the best, and wet mount showed the least performance for the laboratory diagnosis of intestinal parasitic infections.
Highlights
Even if the prevalence of intestinal parasites is high in Ethiopia, we still use only direct wet mount method for laboratory diagnosis of intestinal parasitic infections, having low sensitivity, and this significantly increases false-negative results
Negussu et al [9] reported that, in Ethiopia, the number of people living with STHs in endemic areas is estimated at 79 million, comprising 9.1 million pre-school aged, 25.3 million school-aged, and 44.6 million adults
Wet mount method has been still used as a diagnostic method at all levels of health facilities in Ethiopia
Summary
Even if the prevalence of intestinal parasites is high in Ethiopia, we still use only direct wet mount method for laboratory diagnosis of intestinal parasitic infections, having low sensitivity, and this significantly increases false-negative results. It is estimated that about 3.5 billion people in the world are infected with intestinal parasites [2] They are more prevalent among school children as compared to the general population [3,4]. Negussu et al [9] reported that, in Ethiopia, the number of people living with STHs in endemic areas is estimated at 79 million, comprising 9.1 million pre-school aged, 25.3 million school-aged, and 44.6 million adults. Despite this prevalence, wet mount method has been still used as a diagnostic method at all levels of health facilities in Ethiopia. Evaluating additional laboratory diagnostic methods for intestinal parasitic infections that has not been used before in the country is mandatory as discussed in subsequent paragraphs below
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