Abstract
The organic peracids, such as perbenzoic, performic and peracetic acids, are strong oxidizing agents. It may be considered that they are derived by the inter action of hydrogen peroxide and an organic acid with the formation of an acid peroxide. Thus, the production of peracetic acid may be illustrated as follows: CH.COOH + H2O2 * CH3COOOH + H 20 glacial acetic peracetic acid acid The present paper deals first with the known chemical reactions of organic peracids with organic compounds. This provides one with a background for understanding the chemical basis of the peracetic acid-Schiff reaction in tissue sections. This histochemical method then is described, followed by the results of the method in staining selectively various tissue elements. The use of block ing and extraction procedures and of additional staining reactions are presented in order to substantiate the chemical basis of the peracetic acid-Schiff staining in tissue sections.
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