Abstract
Two electrophoretically and serologically distinct viruslike particles (PsV-f and PsV-s) were isolated from Penicillium stoloniferum Thom. ATCC No. 14586. They were purified by rate-zonal sucrose density-gradient centrifugation and separated by sucrose density-gradient electrophoresis at pH 8.0. Both preparations of viruslike particles were composed of several centrifugal and density components. Electron micrographs contained polyhedral particles with diameters of 34 nm. The viruslike particles with the highest electrophoretic mobility (PsV-f) contained three distinct classes of double-stranded RNA with molecular weights of 0.99, 0.89, and 0.23 × 10 6 daltons as well as single-stranded RNA. The PsV-s contained two double-stranded RNA classes with molecular weights of 1.01, and 0.94 × 10 6 daltons. All of twelve cultures grown from single spore isolates were found to contain both types of virus-like particles.
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