The Pd-catalyzed precise synthesis of self-assembled fluorescent nanoprobes with different molecular weights for enhancing the permeability of cell membrane and targeting lysosomes

Abstract

As a common polymer, poly (dimethylaminoethyl methacrylate) (PDMAEMA) is mainly used to synthesize medical materials. However, there are few reports about the related polymer fluorescent probes. Moreover, as far as we know, no one has studied the effect of its molecular weight on the performance of polymer probes. In this work, polymer probes (NapNH2(1)-PDM(4), NapNH2(1)-PDM(9), NapNH2(1)-PDM(23), NapNH2(1)-PDM(34)) with different molecular weights were accurately constructed based on 1,8-naphthalimide fluorophore and poly(N,N-dimethylaminoethyl methacrylate) and their photophysical properties were systematically studied. The test results showed that they all showed good photophysical properties. Moreover, the polymer probe NapNH2(1)-PDM(23), which had the best self-assembly effect and good cell permeability, was successfully screened by confocal fluorescence imaging technology. Not only that, the probe NapNH2(1)-PDM(23) could also label lysosomes in cells quickly and accurately. Therefore, this work provided important reference to construct relevant polymer probes with good cell permeability in the future.