Abstract
SUMMARY The secretion of progesterone from the autotransplanted ovaries of five ewes was measured by a competitive protein-binding technique. Luteinizing hormone (NIH-LH-S14) increased the secretion rate of progesterone from the ovary when infused for 60 min via the ovarian artery at a rate of 1000 μg/h. The pattern of response in nine experiments was characterized by a significant increase in progesterone secretion which occurred 10–20 min after starting the infusion (150%). Between 30 and 60 min the secretion rate fell progressively towards the control values in spite of the continued infusion of LH. There was a further significant fall in the 30 min following cessation of LH. In three out of nine infusions there was a progressive increase in the ovarian blood flow throughout the infusion with maximal levels being found at the end of the 60-min infusion period.
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