The pathogenetic basis for cell therapy of post‐resuscitation disease, treatment and prevention (912.2)

Abstract

Aim of this study was to improve the effectiveness of resuscitation and to develop the basis for cell therapy and transplants in experimental studies.Material and methods. We used 357 rats. In total 429 experiments were performed. Clinical death and resuscitation was performed by L.Z. Tell’s technique (1982). Resuscitation procedures included transplantation of isolated cells from liver, spleen and thymus from heterogeneous and genetically identical rats and their fetuses. The complex of biochemical, neurological studies was performed to study the influence of cell transplants on post resuscitation disease natural history.Results and discussion: General conclusion of our studies is that use of different cell therapy techniques could change reactivity and resistance of resuscitated animals. As it was shown previously, after resuscitation the non‐specific changes of metabolism are started. Cell therapy generally improves these changes. Metabolic processes including all branches and enzymes activity change after cell therapy to more optimal level. Most important factor of cell therapy was brain cognitive function improvement in resuscitated animals who underwent cell transplant in early after resuscitation period. Optimization of rats behavior and reflexes improvement were evident. This effect was especially marked if fetal cells from genetically identical fetuses were used.