The participation of signal transduction and gene regulation mechanisms in the cascade of MAPK p38 (mitogen-activated protein kinases) in the repair of damage to the serous membrane of the abdominal cavity

Abstract

Objective: to assess the activity of р38 МАРК in serosal injury.Material and methods. We modelled adhesive process in abdomen in 40 Wistar rats. Adhesion zone was immunohistochemically stained for p38 MAPK and p38 MAPK Phospho. Expression of р38 МАР-coding genes was studied the in the injured zone using RT PCR. The control group consisted of 5 intact Wistar rats.Results. As a result of immunohistochemical staining of adhesion zone we determined that peak of p38 expression is registered on the 14th day after surgery, but phosphorylated p38 MAPK activity peak — on the 3rd day. Assessment of p38 MAPK genes expression showed that Mapk12 genes was expression peaks on the 3rd и 14th day, Mapk13 — in 12 hours and on the 7th day, Mapk11 and Mapk14 — on the 14th day. Statistical significance in comparison with data obtained in intact animals (р < 0,05) for MAPK12 was registered on the 3rd day, for MAPK13 — in 12 hours, on the 3rd and 7th day.Conclusion. Our research allowed us to determine р38 МАРК cascade expression in non-changed reparative process. It was found that p38 MAPK cascade activation starts from the 6thhour after the surgery and lasts up to 30th day with its maximum on the 14th day.