Abstract
Summary We have partially purified the mRNA coding for the phenobarbital induced rabbit liver cytochrome P450-LM 2 approximately 90 fold relative to control liver levels. Using a combination of Sepharose 4B chromatography, denaturing and nondenaturing sucrose gradient centrifugation, we have obtained a mRNA that when translated in vitro using wheat germ lysate codes for 7.2% cytochrome P450-LM 2 .
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