Abstract
Kupelwieser(1906)reportedthatthe unfertilizedggsof two species of sea-urchins, Strongylocentrotus purpuratus and Strongy locentrotus franciscanus, exposed to concentrated sperm or to filtrates preparedfrom heatedsperm oftwo species(?)ofchiton, formed fertilization membranes; and, ifsubsequentlytreated with Loeb's hypertonic sea-water, developed. Loeb (1907 and 1908) obtained similar results with the blood serum of different species of animals. In recent experiments I have obtained similar effectswith the ova of S. franciscanus.In addition filtrates preparedfrom suspensionsoflivingsperm of the chiton,Kath anna tunicata, causedtheformationof fertilization membranes in ova of S. franciscanus. Subsequent treatment with hyper tonic sea-water led to the development of many of the ova in which membranes appeared. These experiments were performed during the spring of 1921 at the Hopkins Marine Laboratory at Pacific Grove, California. Iwishtoexpressheremy appreciation ofthehospitality extended to me at thislaboratoryand my thanks to the Director,Dr. Walter K. Fisher,forhisgenerousassistance.
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