Abstract

Ascaris lumbricoides is the most successful geohelminth since it currently infects >1.5 billion people world‐wide. Here,we characterize a readily available source of soluble products in the pseudo‐coelomic fluid (PCF) from adult A. suum. A 42 kDa Ascaris suum hemoglobin (AsHb)was recognized by sera from infected pigs and was identified in the muscle and intestinal tissues and the excretory/secretory products obtained from third and fourth‐stage larvae (L3/L4) and adult worms. Comparative sequence information indicated sequence homology in parasitic but not free living nematodes. Exposure of BMDC to AsHb induced the maturation markers CD40, CD86, and MHCII. Co‐stimulation of BMDC with AsHb and LPS decreased IL‐12 production and significantly increased IL‐10 compared to stimulation with LPS alone. AsHb‐dependent maturation and IL‐10 production were abrogated in BMDC from TLR4‐deficient mice. In addition,confocal studies demonstrate co‐localization of AsHb with TLR4 in mouse BMDC, followed by internalization of AsHb through a receptor‐mediated endocytic pathway. Finally, AsHb was able to remove extracellular free radical reductants produced by activated swine bronchial‐alveolar cells.

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