The Parameter Pharmacokinetics of Caffeine Nanoparticle Preparations from Robusta Coffee Extracted Using PLGA and PVA

Abstract

Coffee is one of the beverages favoured by the public. Coffee contains caffeine which is able to increase concentration and this ability takes place depending on the dose received by the body. The purpose of this study is to isolate caffeine from coffee tree and characterize nanoparticles caffeine in polymerized PLGA-PVA and Its pharmacokinetics (Vd, ke, t1/2 dan Cl) Preparing of caffeine nanoparticles using ingredients form Robusta coffee beans that have been dried and roasted and extracted using digestive method, to obtain caffeine. Then the caffeine is made into a formulation of polylytic nanoparticles PLGA and PVA with emulsion solvent evaporation method. The formulation of caffeine nanoparticles is characterized particle size PSA, zeta potential and morphology is performed by using TEM. In vivo test, using 24 rats in four groups, gave 0,9 mg /200mgBW sample test versus control. Amount 0,5 ml of blood sample test is obtain at time series: 15, 30, 45, 60, 120, 180, 240, 360 and 480 via vena orbitalis to have (ka;Cmax;Tmax:AUC;Vd, ke, t1/2 and Cl) parameters. As the result of characterization is obtained an average diameter of 173.8 nm, zeta potential -16.2 mV and PDI of 0.148. We had Vd is 1954,159 ml. ke is 0,00119 min-1. t1/2 is 580,979 min-1 ; Cl is 2,331 mg/min in Tmax 30 minutes. Its morphology has a spheric surface shape. So that nanoparticle caffeine formulated based Robusta coffee beans can be produced using PLGA and PVA polymers using emulsion solvent evaporation method for topical pharmaceutic and cosmetics proposed.