Abstract
Paeonia qiui is a wild species of tree peony native to China. Its leaves are purplish red from the bud germination to the flowering stage, and anthocyanin is the main pigment in purplish red leaves. However, the anthocyanin synthesis regulation mechanism in tree peony leaves remains unclear. In this study, an R2R3-MYB, PqMYB113 was identified from the leaves of P. qiui. Phylogenetic analysis revealed that PqMYB113 clustered with Liquidambar LfMYB113 and grape VvMYBA6. Subcellular location analysis showed that PqMYB113 was located in the cell nucleus. The transient reporter assay suggested that PqMYB113 was a transcriptional activator. The overexpression of PqMYB113 in Arabidopsis thaliana and tobacco (Nicotiana tabacum) resulted in increased anthocyanin accumulation and the upregulation of CHS, F3H, F3’H, DFR, and ANS. The dual luciferase reporter assay showed that PqMYB113 could activate the promoters of PqDFR and PqANS. Bimolecular fluorescence complementation assays and yeast two-hybrid assays suggested that PqMYB113 could form a ternary MBW complex with PqbHLH1 and PqWD40 cofactors. These results provide insight into the regulation of anthocyanin biosynthesis in tree peony leaves.
Highlights
Anthocyanins are widely distributed in plant flowers, fruits and other tissues (Jaakola, 2013)
Many R2R3-MYB transcription factors that positively regulate anthocyanin biosynthesis have been identified from various plants, such as AtMYB75 (PAP1), AtMYB90 (PAP2), AtMYB113, and AtMYB114 in Arabidopsis (Stracke et al, 2007; Gonzalez et al, 2008), PhAN2 and PhAN4 in Petunia hybrid (Albert et al, 2011), MdMYB1, MdMYBA, MdMYB10, and MdMYB110a in apple (Takos et al, 2006; Ban et al, 2007; Espley et al, 2007; Chagne et al, 2013), PpMYB10.1, PpMYB10.2, and PpMYB10.4 in peach (Rahim et al, 2014; Zhou et al, 2016)
The results suggested that PqMYB113 could interact with PqbHLH1 but not with PqWD40, and PqbHLH1 could interact with PqWD40
Summary
Anthocyanins are widely distributed in plant flowers, fruits and other tissues (Jaakola, 2013). Many R2R3-MYB transcription factors that positively regulate anthocyanin biosynthesis have been identified from various plants, such as AtMYB75 (PAP1), AtMYB90 (PAP2), AtMYB113, and AtMYB114 in Arabidopsis (Stracke et al, 2007; Gonzalez et al, 2008), PhAN2 and PhAN4 in Petunia hybrid (Albert et al, 2011), MdMYB1, MdMYBA, MdMYB10, and MdMYB110a in apple (Takos et al, 2006; Ban et al, 2007; Espley et al, 2007; Chagne et al, 2013), PpMYB10.1, PpMYB10.2, and PpMYB10.4 in peach (Rahim et al, 2014; Zhou et al, 2016) In addition to these activators, some MYB repressors were found, such as FaMYB1 in Fragaria × ananassa (Paolocci et al, 2011), VvMYBC2-L1/L2/L3 and VvMYB4-like in Vitis vinifera (Cavallini et al, 2015), PtrMYB182 in Populus spp. Most of these studies focus on anthocyanin biosynthesis regulation in flowers or fruits, and the molecular mechanism of red color formation in leaves is still not well known
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