Abstract
The human P2Y 1 purinoceptor has been expressed in Jurkat cells and the effects of HPLC purified nucleotides on calcium movements were measured. The most potent agonist was 2-methylthio-ADP followed by ADP. ATP, Sp-ATPαS and β,γ-methylene-ATP were competitive antagonists. Suramin and PPADS inhibited the effects of ADP. This pharmacological profile is the same as that of the so-called P2T purinoceptor responsible for platelet aggregation, which has not yet been identified. Using PCR we found the P2Y 1 receptor to be present in blood platelets and megakaryoblastic cell lines. These data suggest that the P2Y 1 receptor may be the elusive P2T receptor. © 1997 Federation of European Biochemical Societies.
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