Abstract
Pseudomonas aeruginosa is becoming an increasingly prevalent pathogen, capable of causing numerous life threatening infections in immunocompromised patients. The three hierarchically arranged quorum sensing (QS) systems, namely las, rhl, and pqs play key roles in coordinating virulence expression in P. aeruginosa. However, the regulatory mechanisms of the pqs system have not been fully elucidated. To identify new genes involved in synthesis of the Pseudomonas quinolone signal (PQS), a transposon mutagenesis library was constructed. PA1429 was found to inhibit PQS biosynthesis. The PA1429 deletion mutant also exhibited increased bacterial motility, biofilm formation, and virulence in a mouse model of acute lung infection. Interestingly, it also displayed reduced tolerance to oxidative stress. Mutated pqsH in the PA1429 deletion background restored bacterial susceptibility to H2O2. In addition, our data showed that PA1429 repressed the expression of las and rhl systems. Overall, these results provide new insights into the complex regulatory networks of quorum-sensing and virulence expression in P. aeruginosa.
Highlights
Pseudomonas aeruginosa is an opportunistic human pathogen causing a wide range of infections in wounds, burns, and immunocompromised patients
To identify new genes involved in synthesis of Pseudomonas quinolone signal (PQS), a transposon mutagenesis library was constructed and PA1429 was found to inhibit PQS biosynthesis
We identified that inactivation of PA1429 in P. aeruginosa resulted in increased expression of pqsH and in overproduction of PQS
Summary
Pseudomonas aeruginosa is an opportunistic human pathogen causing a wide range of infections in wounds, burns, and immunocompromised patients. The deletion of PA1429 resulted in increased bacterial motility, biofilm formation, and virulence in a mouse model of acute lung infection. To identify new regulators affecting pqsH expression, a transposon insertion mutant library of P. aeruginosa PAO1 carrying the reporter pqsH-lux on its chromosome was constructed. Among the 11 transposon mutants, the PA1429 mutation resulted in significant increase in pqsH expression.
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