Abstract
Tryptamine solutions were incubated with crude suspensions of rat and guinea pig tissues in the Barcroft-Warburg apparatus. Oxygen consumption was measured. Solutions incubated for various periods of time were deproteinized with perchloric acid, and the supernatant fraction was examined in the Beckman DU spectrophotometer. Consistent and marked changes were found in the ultraviolet absorption spectrum of the tryptamine solutions. The quantitative changes have been utilized for the measurement of monoamine oxidase activity by the "substrate disappearance" method. The significance of the qualitative changes in absorption spectrum is discussed. Similar experiments with 5-hydroxytryptamine are presented. This substrate is oxidized by rat liver at about the same rate as tryptamine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.