Abstract
The Oxidation of Thiocyanate by a Cytoplasmic Particulate Fraction of Thyroid Tissue
Highlights
Methods35S-metabolites were characterized by ascending paper chromatography and paper electrophoresis by methods described previously [5, 10]
The oxidation of thiocyanate by this cytoplasmic particulate system is inhibited by the thyroid 105,000 x g supernatant (Table II)
This report describes a cytoplasmic particulate system derived from thyroid tissue that oxidizes thiocyanate, resulting in the formation of inorganic sulfate and a volative %Z-labeled compound
Summary
35S-metabolites were characterized by ascending paper chromatography and paper electrophoresis by methods described previously [5, 10]. Ascorbic acid was determined by the method of Maickel [11]. Analyses were done by means of the biuret [12]. Absorption spectra were recorded with a Cary model 14 spectrophotometer
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