Abstract
Bacteria isolated from the intestinal contents from rats have been found to fix significant quantities of molecular tritium. The possibility that bacteria were the principal agent in the fixation of tritum within the animal body was investigated. Oxidation of tritium by tissue homogenates revealed that oxidation occurred with homogenates prepared from the spleen and intestines. Fixation by the intestinal homogenates was undoubtedly due to bacterial action. A decrease in the bacterial flora of intact animals by either evisceration or treatment with antibiotics reduced the total quantity of tritium oxidized. These results indicate that the bacterial content may be the principal agent in the oxidation of tritium within the animal body.
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