Abstract
The effect of 39 amino acids on the oxygen uptake of Tetrahymena pyriformis W was determined. Respiration was stimulated, in decreasing order of activity, by l-phenylalanine, l-tyrosine, l-cysteine, d-cysteine, l-proline, l-isoleucine, 1,1- dl-aminopropanesulfonic acid, l-leucine, and d-leucine. The effects of varying concentrations of l-phenylalanine and l-cysteine and mixtures of amino acids on respiration were also determined. No amino acid oxidase activity could be detected in cell-free homogenates using l-cysteine, l-phenylalanine, or l-alanine as substrates. Studies were made on the disappearance of l-phenylalanine in relation to oxygen consumption and ammonia production.
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