Abstract
Abstract An oxidation of 2-naphthol by horseradish peroxidase and hydrogen peroxide was studied using fluorescence techniques. 2-Naphthol is fluorescent at a pH of 8, while the product of the reaction is nonfluorescent. The product was identified as 1, 1′-bi-2-naphthol by comparison of its uv spectrum to the standard, commercially available 1,1′-bi -2-naphthol. Similarly, oxidations of 1-naphthol and 2, 7-dihydroxy-naphthalene were studied. The kinetics of these oxidation reactions followed the Michaelis rate equation. The Michaelis constant, Km, was determined by a Lineweaver-Burk plot for each substrate. From the Km values, it was concluded that 2-naphthol is the best substrate (lowest Km value) as compared to all other substrates previously studied by these authors.
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