Abstract
Summary: Hyphomicrobium sp. were grown on ethanol, acetate, 3-hydroxybutyrate and methanol. The specific activities of the following enzymes were measured in cell-free extracts of Hyphomicrobium strain x: ethanol dehydrogenase, acetaldehyde dehydrogenase, acetothiokinase, 3-hydroxybutyrate dehydrogenase, β-keto thiolase, citrate synthase, aconitate hydratase, isocitrate dehydrogenase, α-oxoglutarate dehydrogenase, succinate dehydrogenase, fumarate hydratase, malate dehydrogenase, isocitrate lyase, malate synthase, malate dehydrogenase (decarboxylating), phosphoenolpyruvate carboxylase, pyruvate kinase, phosphoenolpyruvate carboxykinase, phosphopyruvate hydratase and phosphoglycerate mutase. From a comparison of the specific activities of these enzymes in the different cell-free extracts, it was concluded that during growth of Hyphomicrobium sp. on either ethanol, acetate or 3-hydroxybutyrate, energy and reducing power is generated mainly by the tricarboxylic acid (TCA) cycle. Carbon is assimilated via the glyoxylate cycle with phosphoenolpyruvate carboxykinase functioning as the main gluconeogenic enzyme. The conversion of ethanol, acetate and 3-hydroxybutyrate to acetyl-CoA occurs by pathways previously reported in other micro-organisms.
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