Abstract
This study presents the oocyte development of Poecilimon ataturki Ünal, 1999 (Orthoptera, Tettigoniidae) with histology, morphology, and histochemistry by using a stereomicroscope, a light microscope, a scanning electron microscope, and a transmission electron microscope. The ovary in this species is a panoistic type which contains many ovarioles which consist of terminal filament, germarium, and vitellarium. Germarium is the region that has undifferentiated cells which generate the oocytes and follicular cells. In the vitellarium region, yolk granules start to cover the whole oocyte. In histochemical studies, to determine the content of the yolk granules, proteins, and carbohydrates in oocytes were treated with a bromophenol blue (BPB) method, a mercury bromophenol blue (mBPB) method, and a periodic acid Schiff (PAS) method, respectively. As a result of these methods, the yolk granules gave positive results in ovariole sections treated with the PAS and the BPB, while the mBPB staining was negative.
Highlights
The female reproductive system in insects consists of a pair of ovaries which consist of ovarioles, a pair of lateral oviducts, and a genital chamber
Besides all these main reproductive organs such as spermatheca and reproductive glands, there are some other auxiliary structures that help reproduction in female insects which play a role in transfer and protection of the gametes (Nandchahal, 1972; Giuffrida et al, 1996; Sturm & Pohlhammer, 2000; Lange, 2009; Brundo et al, 2011; Sturm, 2012; Masci et al, 2015; Pappalardo et al, 2016)
They were stained with Periodic Acid Schiff (PAS) to mark neutral mucosubstances, and Bromophenol Blue (BPB) and Mercury Bromophenol Blue were used to reveal the protein in the oocytes
Summary
The female reproductive system in insects consists of a pair of ovaries which consist of ovarioles, a pair of lateral oviducts, and a genital chamber. Each ovary continues with a canal called a lateral oviduct where the mature egg moves forward to be left out and the lateral oviducts come together to form a common oviduct of ectodermal origin (Viscuso et al, 1999; Lange, 2009; Klowden, 2013; Pappalardo et al, 2016) Besides all these main reproductive organs such as spermatheca (receptaculum seminis) and reproductive glands, there are some other auxiliary structures that help reproduction in female insects which play a role in transfer and protection of the gametes (Nandchahal, 1972; Giuffrida et al, 1996; Sturm & Pohlhammer, 2000; Lange, 2009; Brundo et al, 2011; Sturm, 2012; Masci et al, 2015; Pappalardo et al, 2016).
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