Abstract
The biocompatibility of the bone implants is a crucial factor determining the successful tissue regeneration. The aim of this work was to compare cellular behavior and osteogenic properties of rat adipose-derived multipotent stromal cells (ASCs) and bone marrow multipotent stromal cells (BMSCs) cultured on metallic substrate covered with TiO2 sol-gel-derived nanolayer. The morphology, proliferation rate, and osteogenic differentiation potential of both ASCs and BMSCs propagated on the biomaterials were examined. The potential for osteogenic differentiation of ASCs and BMSCs was determined based on the presence of specific markers of osteogenesis, that is, alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCL). Additionally, the concentration of calcium and phosphorus in extracellular matrix was determined using energy-dispersive X-ray spectroscopy (SEM-EDX). Obtained results showed that TiO2 layer influenced proliferation activity of ASCs, which manifested by shortening of population doubling time and increase of OPN secretion. However, characteristic features of cells morphology and growth pattern of cultures prompted us to conclude that ultrathin TiO2 layer might also enhance osteodifferentiation of BMSCs. Therefore in our opinion, both populations of MSCs should be used for biological evaluation of biomaterials compatibility, such results may enhance the area of investigations related to regenerative medicine.
Highlights
The biocompatibility of bone implants is a crucial factor determining the successful tissue regeneration
We developed and analyzed the 316 L austenitic steel implant covered with TiO2 layer using sol-gel method
The purpose of this work was to determine the osteogenic properties of both adipose-derived multipotent stromal cells (ASCs) and bone marrow multipotent stromal cells (BMSCs) cultured on 316 L stainless steel-based biomaterial covered with TiO2 sol-gel layer
Summary
The biocompatibility of bone implants is a crucial factor determining the successful tissue regeneration. The stainless steel 316 L grade is most commonly used material in modern orthopaedics and dentistry. It is often applied as plates or nails, used preferably due to the proper mechanical properties and because of its low cost [1]. We developed and analyzed the 316 L austenitic steel implant covered with TiO2 layer using sol-gel method. These results clearly demonstrated biocompatibility of obtained
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