Abstract
This research was conducted to determine the closed transportation system with biomass and water volume ratio that can support the osmoregulation ability of glass eel significantly. Glass eel was obtained from catching activities in the Cimandiri River, Pelabuhan Ratu (mean length 52.40+0.84 mm and weight 0.10+0.01 g) and acclimatized for 24 hours at salinity 6 mg L-1 and temperature 20°C gradually.The research design was a completely randomized design. The applied treatment was glass eel closed transportation system with various ratio of glass eel biomass (kg): water volume (L), i.e. 1:11; 1:12; 1:13; and 1:14 during 24 hours of land transportation and continued with a 3-day recovery period.The water was used refer to the above acclimatization method, with ratio the water part of pure oxygen in a plastic bag was 1:3. The measured parameters include survival rate, osmotic gradient, body hydromineral, and water quality in transport packaging and recovery medium. Survival rates were generated in the high range until recovery period (P<0.05), but the transportation method caused differences significantly (P<0.05) in osmotic response and hydromineral status (sodium, chloride, potassium, and water content), so that it will affect production performance in the next stage of culture. Ratio of glass eel biomass: water volume of 1:13 has been able to support the ability of osmoregulation glass eel to maintain homeostatic condition during the transportation until 3 days of recovery period. There was no drastic decline in physical and chemical value of water in the transport bag so it could reduce the mortality rate for 24 hours.
Highlights
The transport method of glass eel, A. bicolor bicolor, in Indonesia generally still refers to closed system for various types of fish larvae and shrimp seed
The common procedures used for live fish transportation was 24 to 168 hours fasting condition, which depend on species of fish, temperature, density, transportation system, unloading packaging, and post transportation handling (Boerrigter et al, 2013)
The convenience of transporting aquatic organisms is an important issue of live fish transportation activities, especially on export destination countries of eel originating from Indonesia, one of which relates to the technical documentation of mortality during the transportation of aquaculture organisms and all its products between and or out of the cultivation location
Summary
The transport method of glass eel, A. bicolor bicolor, in Indonesia generally still refers to closed system for various types of fish larvae and shrimp seed. Glass eels stadium is susceptible to stress that can generate high mortality right after transporting, on arrival and recovery period This is related to the condition of glass eel in poor performace because of the lack of transport method that can not support physiological functions during the transportation. Studies and publications on the transportation of post captured A. bicolor bicolor glass eel in the salinity medium for a quite a long time duration without substitution of water and the addition of pure oxygen are still very limited, especially the influence on the osmotic response and the hydromineral dynamics of the body that can support maximum survival. This research needs to be done with the aim of determining the transport method of A. bicolor bicolor glass eel with an appropriate ratio of biomass and water volume, so that it can support osmoregulation activity during transportation and recovery period
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