Abstract
The examination of peritoneal fluid obtained by paracentesis and peritoneal lavage has contributed to the evaluation of the patient with abdominal injury [5,7]. Quantitation of erythrocytes and leukocytes, and the identification of bacteria can accurately establish the presence of injury or inflammatory and circulatory disorders involving intraperitoneal organs [6, 81. The analysis of peritoneal fluid for its amylase content is the only chemical investigation that has been regularly utilized in the diagnosis of abdominal trauma [4] In an effort to extend the value of paracentesis as a diagnostic tool, the chemical composition of the peritoneal fluid following abdominal trauma has been studied in a clinical and experimental series. The results of the chemical analysis of the peritoneal fluid in a series of 70 patients has been reported [l]. The study found that a combination of an elevated peritoneal fluid alkaline phosphatase activity and a normal serum alkaline phosphatase activity was seen in patients with full-thickness small intestinal injury or a combination of a small intestinal injury and other organ injuries. The present report is an experimental confirmation of this finding with isoenzyme analysis of the peritoneal fluid alkaline phosphatase to determine the source of this alkaline phosphatase activity.
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