The organization of thalamic projections to the parietal cortex of the Virginia opossum.

Abstract

The thalamic projections to somatic sensory-motor (SSM) cortex and adjacent cortical areas of the Virginia opossum were studied using anterograde and retrograde axoplasmic transport techniques. Large injections of horseradish peroxidase and/or tritiated amino acids were made in the parietal cortex to identify all of the thalamic nuclei that are interconnected with this large cortical area. Very restricted injections were then made in physiologically identified subdivisions of SSM cortex, in the remaining posterior portion of parietal cortex, and in the anteriorly adjacent postorbital cortex. The results show that the parietal cortex is reciprocally connected with a number of thalamic nuclei. Different combinations of these thalamic areas project to specific subregions within the parietal field. All parts of the SSM cortex, which occupies the anterior four-fifths of parietal cortex, receive input from the ventrobasal complex (VB), the ventrolateral complex (VL), the central intralaminar nucleus (CIN), the central lateral nucleus (CL), and the ventromedial nucleus (VM). We could detect no segregation of VL and VB inputs in any part of SSM cortex. Projections from all of these thalamic nuclei, except VM, show at least some degree of topographic organization. Anterior-posterior strips of SSM cortex receive input from clusters of thalamic neurons that extend dorsoventrally and rostrocaudally through VB and VL. The posterior one-fifth of the parietal cortex (the posterior parietal area) receives input from VL, the posterior nuclear complex, and the lateral complex, as well as input from CL, CIN, and VM. Postorbital cortex receives input mainly from intralaminar, midline, and medial thalamic nuclei. We conclude that the projection field of VB in the parietal cortex coincides precisely with the first somatic sensory area (SI) as defined by single unit studies (Pubols et al., '76). The VB projection field also delineates the area of the first motor (MI) representation. Thus, there is no separation of SI and MI cortex in the opossum. The posterior parietal area lies outside of SSM cortex and has thalamic connections similar to the posterior parts of parietal cortex in other mammals.