Abstract
In recent years, imaging systems have increasingly become more powerful and have allowed for better spatial resolution. Such systems, however, are limited to surface level interrogation of samples. Here we have developed a general optical technique referred to as Optical Recognition of Constructs Using Hyperspectral Imaging and Detection (ORCHID) as means of obtaining a multidimensional image containing information in the three spatial (X, Y, and Z) and spectral dimensions. Spatial offsets, obtained using selected binning of radially positioned pixels on the CCD, are coupled with stage and spectral scanning to collect a hyperdimensional data cube of the sample. We demonstrate this technique on gel phantoms containing target nanoparticles such as gold nanostars and quantum dots as way to demonstrate in-depth 3D imaging.
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