Abstract
Lotus (Nelumbo nucifera Gaertn), a kind of perennial aquatic plant, is widely cultivated and consumed by people in Asian countries. Lotus plumule flavonoids (LPF) have been recognized as a hypoglycemic agent. LPF was optimally obtained using novel ultrasonic-microwave assisted synergistic extraction (UMSE) method by response surface methodology (RSM) on the basis of the results of single-factor experiments. Furthermore, the hypoglycemic activity of LPF was investigated by measuring the body weight, fasting blood glucose (FBG) level, and oral glucose tolerance test (OGTT) and analyzing the physiological indexes in streptozotocin-diabetic mice model. The optimum extraction conditions consisted of microwave power 355 W, ultrasonic power 423 W, extraction time 15 min, solid-liquid ratio 1:40, ultrasound/interval time 1/0, and ethanol concentration 70% with the maximum LPF yield of 2.62%. LPF supplementation significantly decreased the body weight, FBG, OGTT, serum total cholesterol (TC), serum total triglycerides (TG), and insulin levels, indicating the antidiabetic activity of LPF. This research verified that the UMSE technique was highly efficient to extract LPF to the maximum extent and the flavonoids from L. plumule exhibited hypoglycemic activity, which showed broad development and application prospects.
Highlights
Diabetes mellitus (DM), a chronic metabolic disorder, is characterized by hyperglycemia syndrome and subsequently caused co-morbidities syndrome including hypertension, hyperlipidemia, and cardiovascular diseases (Rubio et al 2008; Wan et al 2020)
The extract solution appeared bright green color and the Lotus plumule flavonoids (LPF) sample was light in color in the treatment of 90% ethanol concentration during the experiment, which may be caused by excessive dissolution of chlorophyII
The results were expressed as mean ± standard deviation (SD) (n = 8/group)
Summary
Diabetes mellitus (DM), a chronic metabolic disorder, is characterized by hyperglycemia syndrome and subsequently caused co-morbidities syndrome including hypertension, hyperlipidemia, and cardiovascular diseases (Rubio et al 2008; Wan et al 2020). A variety of oral anti-diabetic agents such as biguanides, sulfonylureas, α-glucosidase inhibitors, and dietary glucose regulators could be used in clinical treatment, controlling blood glucose within in normal range (Dall et al 2014; Natalia & Montori, n.d.) These medicines might cause toxicity and serious side effects in a long-time cure (Vasconcelos et al 2011). The bioactive effects including antioxidant and anti-inflammatory properties from L. plumule flavonoids (LPF) were well reported (Chen et al 2019), systematic studies on their hypoglycemic activity remain elusive. Response surface methodology (RSM) is an effective statistical technique which optimizes the multiple parameters It is used for designing experimental experiments, modeling the extraction variables, verifying the statistical significance, and obtaining the optimum conditions of extracting LPF (Alara et al 2017). The hypoglycemic activity of LPF was investigated by measuring the body weight, FBG level, and OGTT and analyzing the physiological indexes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
