Abstract
The locally isolated Pseudomonas aeruginosa NW was tested for their ability to produce alcohol dehydrogenase (ADH) enzyme by growing the bacteria on three different aromatic hydrocarbons: naphthalene, xylene and toluene. Following five days incubation the bacterium showed a good ability to utilize xylene, limited ability for toluene utilization and failed to degrade naphthalene. The ADH activity was assayed following each day of incubation on xylene as sole source of carbon and energy and it was found that a maximum ADH volume activity was 2.86 and 4.11 U/ml following the third and fourth day of incubation, respectively. The production of ADH was optimized under different conditions involving: temperature, pH, xylene concentration and incubation period. Results indicated that the optimal conditions for ADH production were by incubating the bacterium in MSM medium containing 1% xylene, pH: 8 for four days and incubation at 37ºC.
Highlights
The locally isolated Pseudomonas aeruginosa NW was tested for their ability to produce alcohol dehydrogenase (ADH) enzyme by growing the bacteria on three different aromatic hydrocarbons: naphthalene, xylene and toluene
Aromatic Hydrocarbon Utilization To investigate the effect of carbon source on ADH production, P. aeruginosa NW cells were grown in MSM media containing 0.2% of different aromatic hydrocarbons as a carbon source
Enzymatic assay showed that ADH is one of group of enzymes which involved in aromatic hydrocarbon oxidation and dehydrogenation of benzyl alcohol and benzaldehyde and their m and p-methyl derivatives are induced only during the growth on the hydrocarbons, the alcohols or the aldehydes [14]
Summary
The locally isolated Pseudomonas aeruginosa NW was tested for their ability to produce alcohol dehydrogenase (ADH) enzyme by growing the bacteria on three different aromatic hydrocarbons: naphthalene, xylene and toluene. 3. Effect of temperature: An overnight culture of P. aeruginosa NW (O.D. 0.6) were grown in MSM, pH: 7.0 media containing 1% xylene with shaking at different temperature values (30, 35, 37, and 40°C) for 4 days incubation.
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