Abstract
Background One of the most important unit operations in upstream animal cell bioprocesses at scales over 100 L is the preparation and sterilization of the medium. This complex, sensitive, and expensive process requires a considerable investment in both material and time [1]. Traditionally, large-scale medium sterilization is performed with costly single-use dead-end filters. To optimize and reduce the cost of this unit operation, we investigated the sterilization of mammalian cell culture medium at volumes larger than 100 L.
Highlights
One of the most important unit operations in upstream animal cell bioprocesses at scales over 100 L is the preparation and sterilization of the medium
Our results indicate that a bearingless centrifugal pump could provide twice the flow rate at the desired filtration pressure in comparison to a peristaltic pump
The bearingless centrifugal pump was able to provide a constant flow in comparison to the peristaltic pump
Summary
One of the most important unit operations in upstream animal cell bioprocesses at scales over 100 L is the preparation and sterilization of the medium. This complex, sensitive, and expensive process requires a considerable investment in both material and time [1]. Large-scale medium sterilization is performed with costly single-use dead-end filters. To optimize and reduce the cost of this unit operation, we investigated the sterilization of mammalian cell culture medium at volumes larger than 100 L
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