The Optimal Time of GLP-2 Intervene for Early Intestinal Rehabilitation in a Rat Model of Massive Bowel Resection

Abstract

Introduction: To explore the optimal time of GLP-2 intervene for early intestinal rehabilitation in a rat model of massive bowel resection. Methods: The rats underwent massive bowel resection (80 % small bowel resection) were divided into two groups: glucagon-like peptide-2 (GLP-2) and saline were given introperitoneal injection for 3 days at different time points following by MBR, respectively. The expression of apoptosis was detected by Western Blotting by detecting the apoptosis-related protein PARP and Caspase 8. Resuts: The number of autophagic body was significantly reduced after the treatment of GLP-2 at postoperative 24 hours. The expression of autophagy marker including LC3, Atg5 and Beclin-1 was heavily reduced after the treatment of GLP-2 at postoperative 24 hours on the level of mRNA. Among them, the level of Beclin-1 was the most significantly reduced. While the expression of P62 significantly increased after the treatment at this time point. The rate of transition from LC3 I to LC3 II and the expression of LC3 II were reduced significantly after the treatment of GLP-2 at postoperative 24 hours. Meanwhile, the levels of Atg5 and Beclin-1 were also reduced at this time point. However, the levels of P62 decreased significantly after the treatment. The expression of autophagy increased significantly after the treatment of GLP-2 at postoperative 72 hours. Conclusion: The intervene of GLP-2 initiated at postoperative 24 hours is the optimal time for early intestinal rehabilitation a rat model of massive bowel resection.