The optimal of xylanase production conditions using empty fruit bunch raw biomass by marine bacterium Bacillus sp LBF-001

Abstract

Several xylanases have been studied recently, but few xylanases are from marine microorganisms have been reported. Marine bacterium Bacillus sp. LBF-001 was isolated from Pari Island Kepulauan Seribu in Indonesia. The purposes of this study are to identify of 16S rDNA gene from marine bacterium LBF-001 and to optimize medium conditions including kind and concentration of biomass, nitrogen source, pH and temperature. With 16S rDNA gene analysis that LBF-001 isolate have 99% identity with Bacillus pumilus HT-Z4-B2 (KJ526885). Fermentation for producing xylanases was done by using several agricultural residues under solid-state fermentation (SSF). The optimum condition for xylanase production by isolate Bacillus sp. LBF-001 are 2.5% empty fruit bunch, 0.6% lactose broth, pH values was 6.5, temperature condition was 30oC, under submerged fermentation with shaking at 150 rpm for 48 hours fermentation and giving the xylanase activity 10.85 U/mL.