The optic lobe–pars intercerebralis axis is involved in circa’bi’dian rhythm of the large black chafer Holotrichia parallela

Abstract

The large black chafer Holotrichia parallela exhibits ~ 48-h circa'bi'dian rhythm. Although circabidian rhythm is suggested to involve the circadian clock, no physiological studies have been conducted to verify this involvement. We examined the effects of optic lobe or pars intercerebralis removal on the circabidian rhythm. After removing both optic lobes, all beetles lost their circabidian rhythms (N = 25), but all beetles exhibited circabidian rhythm after removing unilateral optic lobe (N = 18). However, 22% of the latter group exhibited day switching. After removal of the pars intercerebralis, 26.3% beetles showed arrhythmic patterns (N = 19). The number of paraldehyde fuchsin-stained pars intercerebralis cells in the arrhythmic group was significantly reduced compared to in the intact and sham-operated groups. The activity in the pars intercerebralis-removed beetles was significantly higher than that in the control groups. The results show that the optic lobe and at least part of the pars intercerebralis are necessary for circabidian rhythm, and bilateral optic lobes are necessary to maintain regularity of the two-day rhythm in H. parallela. This suggests that a neural circuit of circadian clock cells in the optic lobe to pars lateralis might be evolutionally conserved and used also for the generation of circabidian rhythm.