Abstract
Low (1 × 10 −9 M) concentrations of cytochrome c inhibit H 2O 2 production in cytochrome c-depleted mitochondria, purified succinate-cytochrome c reductase (SCR) and antimycin A inhibited cytochrome c-depleted HMP. At higher concentration (2 × 10 −6 M), cytochrome c eliminates pre-existed H 2O 2 if feeding electrons to it by succinate. Cytochrome c also decreases the OH produced by succinate-cytochrome c reductase oxidizing succinate. We conclude that the alternative electron-leak pathway mediated by cytochrome c operates very well. In the presence of antimycin A, ferrocytochrome c can suppress the generation of H 2O 2 in SCR system, but ferricytochrome c cannot. Similar results are obtained on the elimination of pre-existed H 2O 2 by cytochrome c. For hydroxyl radical, antimycin A abolishes the suppression caused by both ferrocytochrome c and ferricytochrome c. These results indicate that the reductive state of cytochrome c caused by electron-flow is necessary and sufficient for the operation of cytochrome c-mediated electron-leakage pathway.
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