The oesophageal diverticulum of Dirioxa pornia studied through micro-CT scan, dissection and SEM studies

Kala Bhandari,Michael A Keller,Peter Crisp

doi:10.1186/s12896-019-0585-8

Abstract

BackgroundDirioxa pornia (Diptera, Tephritidae) (Island fly) is an Australian native species related to a number of pestiferous fruit flies but, unlike many of the pest species, has not been studied extensively due to its non-pest status. However, due to D. pornia’s apparent reliance on the bacteria for survival it is an ideal species to undertake studies into interaction between Tephritid species and bacteria associated with the intestinal tract. The oesophageal diverticulum, which is a blind-ended protrusion of the oesophagus, has been studied, described and characterised in many other Tephritid species. Unlike many other species where the oesophageal diverticulum has been observed the organ was only observed in male D. pornia. It is speculated that this sexual dimorphism the organ may be the primary location to host beneficial bacteria in the involved in the production of the nuptial gift and the mating success of this Tephritid species. In case of D. pornia, however, no study on any area of the digestive system has been conducted. This study was conducted to locate and characterize the oesophageal diverticulum in D. pornia. A virtual dissection of the alimentary tract was made through micro-computer tomography studies. These studies were followed by dissection and scanning microscopy studies to elucidate the presence of bacteria.ResultsThe oesophageal diverticulum of D. pornia is part of the foregut and distends from the oesophagus within the head of the fly. The shape of the oesophageal diverticulum corresponds with the Ceratitis type. Scanning microscopy studies of the oesophageal diverticulum show rod-shaped bacterial cells residing along with yeast cells in the lumen. The organ was only observed in male specimens.ConclusionsThis study classifies the oesophageal diverticulum of D. pornia under the “Ceratitis type” of oesophageal diverticula in Tephritid species. The study also proves that micro-CT scanning is possible to locate soft tissues in Tephritid species and the Avizo® Fire software can be successfully used to visualize 3 dimensional (3D) images from x-rays. The methods used in this experiment can be used in future studies for visualising soft tissues of adult Tephritid species through micro tomography. There is sexual dimorphism with the organ only found in males. Finally this study shows that bacteria are present in the oesophageal diverticulum of D. pornia.

Highlights

Dirioxa pornia (Diptera, Tephritidae) (Island fly) is an Australian native species related to a number of pestiferous fruit flies but, unlike many of the pest species, has not been studied extensively due to its non-pest status
Preliminary dissections During preliminary dissections made on the D. pornia adults, it was noted that no female flies of the 50
This finding has led to another study on the sexual dimorphism in D. pornia, which will be published in future

Summary

Introduction

Dirioxa pornia (Diptera, Tephritidae) (Island fly) is an Australian native species related to a number of pestiferous fruit flies but, unlike many of the pest species, has not been studied extensively due to its non-pest status. The oesophageal diverticulum, which is a blind-ended protrusion of the oesophagus, has been studied, described and characterised in many other Tephritid species. Unlike many other species where the oesophageal diverticulum has been observed the organ was only observed in male D. pornia. This study was conducted to locate and characterize the oesophageal diverticulum in D. pornia. Fruit flies (Diptera; Tephritidae) constitute some of the major pests of horticultural crops Control measures such as the sterile insect technique (SIT) are used to keep populations of many fruit fly species within manageable limits. The oesophageal diverticulum was considered exclusive to the Olive fly until 1973, when Girolami discovered and described the organ in most Tephritid species [11], as follows, a. The Dacus type is the largest of the oesophageal diverticula and the bacteria multiply near the elongated basal epithelial cells

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