Abstract
1. 1. Partially purified extracts from a wide range of insect species were analysed by radio-immunoassay (RIA) using antisera raised against five vertebrate steroids and ecdysone. 2. 2. RIA-positive material was detected in all the extracts with most of the antisera, implying all of these steroids to be of widespread occurrence. 3. 3. The titres of vertebrate-type steroids were over a comparable range to the ecdysteroid titres, indicating that their potential physiological importance should not be ruled out. 4. 4. Further characterization of the RIA-positive material present in some of the extracts was achieved by combining chromatographic techniques with RIA.
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