Abstract
Summary Bacterial plate counts of over 10,000 per milliliter have been obtained from samples of diluted bull semen containing sulfanilamide, penicillin, and streptomycin. These counts were obtained when penicillinase and para amino-benzoic acid were included in the plating medium to inactivate the penicillin and sulfanilamide. Comparison of per cent nonreturns from semen with low and with relatively high counts indicated that these bacteria were not related to fertility. Sulfanilamide had little or no effect on the bacterial content of semen containing penicillin and streptomycin. The inclusion of both of the antibiotics is desirable, however, since bacteria are usually present that are resistant to one but not to the other. The resistant bacteria are primarily diphtheroids and are inhibited but not killed by low concentrations of penicillin; however, they are not inhibited by relatively high concentrations of streptomycin.
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