Abstract
The occurrence of acetylcholine in the heads of houseflies has been demonstrated. After a preliminary purification by paper electrophoresis, acetylcholine was identified by paper chromatography, paper electrophoresis, pharmacological methods, and by its reaction with hydroxylamine – ferric chloride. The base was also isolated as a reineckate, converted to the perchlorate, and its infrared spectrum shown to be identical with reagent acetylcholine.
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