Abstract
The early passage diploid Syrian hamster embryo (SHE) cells were treated with N-methyl- N′-nitro- N-nitroguanidine (MNNG). The treated cells proliferated rapidly; the doubling times shortened; colonies appeared in solid sugar agar medium and transformed foci formed in tissue culture. All of these phenomena suggest that malignant transformation of SHE cell has occured. Faster cell division rate and multipolar mitosis were demonstrated by time-lapse cinemicrography and scanning electron microscopy. Multipolar mitosis can occur in two forms: direct division and indirect division. The transformed cells were more abundant in microvili, the number of which increased in accordance with the degree of malignancy. In comparison with the controls, the transformed cells expressed a greater tritiate thymidine incorporation, greater DNA contenst and more chromosomes, but no difference in nuclear area. The determination of amino acid changes in media due to the growth of transformed cells showed that the decrease in arginine and increse in ornithine are significant. The results of allogenic animal inoculation suggest that the transformed cells can be characterized into several different stages in the process of transformation.
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