Abstract
Heparin was demonstrated by the microelectrophoresis procedure of Jaques & Wollin in endothelial powders obtained after acetone extraction of Häutchen preparations after injection of 1,000 units in rats. Concentrations of heparin calculated for the endothelium was 30 to 7,500 times greater than the concentration of heparin in blood at a given time with a comparable dose. An electron micrograph of preparations stained with Ruthenium Red taken at five minutes after intravenous injection of heparin, demonstrated a marked increase in electron density, as compared to normal endothelium, at the cell membrane and in vesicles near the luminal surface of the cell.
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