The Observation of FSH’s Cellular Internalization

Jizhong Han,Fei Chen,Bin Zeng,Xian Zhang,Lanlan Liu,Jianwen Hu

doi:10.4236/jbm.2016.412006

Abstract

Follicle stimulating hormone (FSH) is a kind of glycoprotein gonadotropin, and plays an important role in the diagnosis and treatment of infertility. Follicle stimulating hormone receptor (FSHR) is a kind of G protein coupled receptor (GPCR), found in the ovary and testes, and its activation is required for the hormonal operation during the breeding period. In this study, an experimental model of FSHR mediated FSH into cell membrane, which exhibited a phenomenon of fluorescent localized on cell surfaces internalized into cell interior, was established to verify biological activity of FSH.

Highlights

Human follicle stimulating hormone, synthesized and secreted by the pituitary gland, is a kind of glycoprotein gonadotropin [1]
Follicle stimulating hormone receptor (FSHR) protein was expressed on the surface of cell membrane of HEK-293 cells transfected with pSNAPf-ADRβ2-FSHR vector
No red fluorescence was obserbered in the cell membrane of the non transfected HEK-293 cells. (Figure 2) It indicated that the fusion protein was on the cell membrane. 3.2

Summary

Introduction

Human follicle stimulating hormone (hFSH), synthesized and secreted by the pituitary gland, is a kind of glycoprotein gonadotropin [1]. FSHα and FSHβ subunits are both involved in receptor binding and signal mediating. The binding of FSHα and FSHβ subunits has a direct effect on the activity of hormones [3]. Separating FSHα subunit and FSHβ subunit will cause the FSH to lose biological activity [4]. Because FSH is a macromolecular protein, it requires receptor follicle stimulating hormone receptor (FSHR) to enter into the cell membrane. Because the purification of Urinary and Pituitary source follicle stimulating hormone is difficult, it Contains other Miscellaneous protein, has certainly side effects. The aim of genetic engineering expressed recombinant human follicle stimulating hormone protein is to explore its medicinal value and to use in clinical research in the future. The cellular internalization of FSH standard sample can provide reference for the expression of rhFSH protein’s activity detection

Targeted Binding Receptor

Cell Staining

Comparison of Transfection and Non Transfection of HEK-293T Cells

Discussion