Abstract
Serological tests revealed immunochemical similarities between the lipopolysaccharides of Hafnia alvei strains PCM 1200, 1203 and 1205. Immunoblotting and ELISA showed cross-reactions between the strains. NMR spectroscopy showed that the O-deacetylated O-specific polysaccharides isolated from lipopolysaccharides of H. alvei strains PCM 1200 and 1203 possessed the same composition and sequence as the O-deacetylated O-specific polysaccharide of H. alvei strain PCM 1205, that is a glycerol teichoic-acid-like polymer with a repeating unit of the following structure: [Display omitted] NMR spectroscopic studies of the polysaccharides concluded that O-3 of the side chain β- d-Glc pNAc is partially O-acetylated (50–80%) in both investigated strains. In strain PCM 1203 an additional O-acetyl group (50–80%) is linked to O-6 of the chain →3)-α- d-Glc pNAc-(1→ residue. The structural features of the isolated O-specific polysaccharides were also the same as those of the O-specific polysaccharides on the bacterial cells directly observed by the HR-MAS NMR technique.
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