THE NUTRITIONAL REQUIREMENTS OF EXCISED ROOTS OF THREE GENOTYPES OF ARABIDOPSIS THALIANA (L) HEYNH.

Abstract

SummaryMethods are described for the culture of excised roots of Arabidopsis thaliana. Cultures of the roots of three genotypes (EST, PI and 1018/6) were established. The genotypes PI and 1018/6 each differ from the wild‐type, EST, by a single gene mutation. It is shown that there are differences between the excised roots of the three genotypes in growth rate and in the requirement for the vitamins, thiamin and biotin. The vitamin requirements of the excised roots largely reflect those of intact plants, as established by Langridge. However, roots of the genotype PI responded to thiamin and biotin, whilst whole plants of this strain respond only to biotin.