Abstract
The effect of cyst(e)ine and sodium chloride concentration on oxygen uptake, and hydrogen sulphide, ammonia, and pyruvic acid production by Vibrio costicolus is described. Added pyridoxal was unnecessary for maximum desulphydrase activity. Generally, oxidation curves of cyst(e)ine concentrations 4 μM or less were the customary type whereas curves which resulted from the oxidation of 6.25 μM cyst(e}ine and greater were of a type indicating accumulation of a product toxic to the system. This occurred in the presence of 1 M NaCl and the suppressed type of oxidation could be returned to normal by increasing the concentration of NaCl. Pyruvic acid accumulated in the flasks showing the suppressed type of oxidation curves, but did not accumulate in flasks showing customary oxidation curves. Pyruvic acid did not accumulate in flasks containing 6.25 μM cyst(e)ine if the sodium chloride concentration was increased to 1.5 M or greater. Pyruvic acid did accumulate with 3.125 μM cyst(e)ine if a citric acid cycle inhibitor (sodium fluoroacetate) was added to the flask contents. These studies suggest that the most likely pathway for the utilization of cyst(e)ine, an essential nutrient for V. costicolus, is its conversion to pyruvate to enter the citric acid cycle.
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