The number and distribution pattern of Golgi bodies in cells of Micrasterias (Conjugates, Chlorophyta) examined by fluorescence microscopy and electron microscopy

Abstract

SUMMARYThe number and the distribution pattern of Golgi bodies in cells of Micrasterias americana and Micrasterias crux‐melitensis were examined both by fluorescence microscopy and by electron microscopy. Golgi bodies intensely absorbed the fluorescent dye DiOC6(3) and strongly radiated fluorescent light. The number of Golgi bodies nearly doubled before septum formation, and half of the Golgi bodies entered each sister cell. Many Golgi bodies migrated from the non‐growing half‐cell to the growing half‐cell where new cell walls were actively being synthesized. Most Golgi bodies were not in contact with chloroplasts in the growing half‐cell. Half of the Golgi bodies moved back to the non‐growing half‐cell 6–12 h after completion of the new half‐cell. Golgi bodies were in contact with the surfaces of chloroplasts 12 h after full growth.