The nucleotide sequence of mannosyl-Q-containing tRNA Asp from Xenopus laevis oocytes

Abstract

The nucleotide sequence of tRNA Asp from X. laevis oocytes was deterd as being: pU—C—C—U—C—m 2G—U—U—m 1G—G—U—A—Ψ—A—G—G—U—G—G—D—G—A—G— U—A—U—C—C—C—C—G—C—C—U—Q 1—U—C—A—m 5C—G—C—G—G—G—A—G— A—m 5C—m 5C—G—G—G—G—T—Ψ—C—A—A—U—U—C—C—C—C—G—A—C—G— G—G—G—A—G—C—C—A OH. The tRNA is 75 nucleotides long. This sequence is very similar (75% to 97% identity) to all other eukaryotic tRNA Asp sequenced so far, except for the bovine liver tRNA Asp (32% identify). The relation between the presence of a mannosyl group on queuosine (Q) at position 34 and the nucleotide sequence of the anticodon loop is discussed.